HPLC Column
Mightysil Series
In the Mightysil series lineup, a C8 and C4 with lower hydrophobicity is also offered mainly with silica gel based reversed-phase fillers, particularly for ODS fillers that are mostly used for general purposes. Including the test items concerning the elution behavior of each filler batch which were implemented in the GP series, additional items by analysis subject according to its application were also implemented for the columns by application.
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Product Lineup
Reversed Phase
| Mightysil RP-18GPⅡ | ODS with di-functional octadecyl sililation. Excellent durability for acidic or basic eluent achieved by fully optimized endcapping and di-functional modification of silicagel. In some case, stereoselectivity will different from monofunctional ODS. |
|---|---|
| Mightysil RP-18GP | High performance, conventional column with monofunctional ODS, you will achieve highly cost performance with GP series. |
| Mightysil RP-18MS (For LC/MS) |
Improved reliably for LC/MS by optimized endcapping, you will achieve lower back pressure, while maintaining high theoretical plates. Moreover, elution of residual silylation agent is drastically reduced, the damage for MS detector is distinctly lowered. |
| Mightysil RP-18GP Aqua | ODS stable retention is achieved with up to 100% water-based eluents. For analysis with high polarity eluent, such as separation of samples with high hydrophilicity. |
| Mightysil RP-8GP Mightysil RP-4GP |
Highly purified spherical silica gel modified with the octyl group and butyl group. You will achieve more retention for substances with a high hydrophobicity samples. |
| Name | Particle Size(μm) | Pore Size(nm) | Modification Group | Carbon Content | Modification |
|---|---|---|---|---|---|
| Mightysil RP-18GPⅡ | 3, 5 | 14 | C18 | 15％ | Difunctional |
| Mightysil RP-18GP | 3, 5 | 12.5 | C18 | 20％ | Monofunctional |
| Mightysil RP-18MS | 5 | 12.5 | C18 | 20％ | Monofunctional |
| Mightysil RP-18GP Aqua | 3, 5 | 13.5 | C18 | 15％ | Monofunctional |
| Mightysil RP-8GP | 5 | 12.5 | C8 | 12％ | Monofunctional |
| Mightysil RP-4GP | 3, 5 | 12.5 | C4 | 4％ | Monofunctional |
Normal Phase
| Mightysil NH2 | Monomeric modified aminopropylsilyl to high purity silicagel. Aminopropyl is often used in the analysis of the saccharide in particular, but is also applied as the weak anion exchange phase and the reverse phase separation which used specific selectivity. |
|---|---|
| Mightysil Si60 | 99.99% over high purity silica gels pore size 60Å for normal phase separation. |
| Name | Particle Size(μm) | Pore Size(nm) | Modification Group | Carbon Content | Modification |
|---|---|---|---|---|---|
| Mightysil NH2 | 5 | 7 | NH2 | Monofunctional | |
| Mightysil Si60 | 5 | 6 |
Flagship Product
Mightysil RP-18 GPⅡ
Mightysil GP II is highly inert ODS stationary phase because of not only di-functional bonding to 2 silanols but also optimized new end-capping methodology which deactivate the residual silanol groups. Thus peak tailing is suppressed to a minimum and you will achieve the highest sensitivity of any samples. Moreover, excellent durability will be accomplished in use of acid-like eluent for it’s excellent inertness of stationary phase.
Excellent Durability
Generally, ODS stationary phase is hydrolyzed by acid, like a trifluoroacetic acid (TFA) solution, and deteriorates the column performance irreversibly. Compared with the monofunctional ODS silica gel, "Mightysil RP-18 GPII" demonstrates excellent durability even under acidic eluents and stable retention times can be obtained. This is declare that "Mightysil RP-18 GPII" is also suitable for LC/MS application which offen use acidic eluent containing TFA, Formic acid, etc.
After a 0.5% TFA solution was supplied to the column for sufficient replacement, the column was sealed and left for a predetermined time at a room temperature of 15 to 20 °C. After a predetermined time, the retention time of the anthracene was measured, and compared with the initial retention time.
- Column： Mightysil RP-18GPⅡ 150-4.6 (5μm)
- Mightysil RP-18GP 150-4.6 (5μm)
- Eluent： Acetonitrile/Water=75/25
- Flow rate： 1.0mL/min
- Temp.： 40℃
- Sample： Anthracene
Mightysil RP-18MS
We suggest the HPLC column "Mightysil MS" which support becoming high sensitivity LC/MS analysis and is used reliably. We improve the conventional "Mightysil GP" series and realize lower back pressure with keeping it’s high resolution.
Higher Number of Theoretical Plate
We succeeded in reducing 10% of column back pressure in using acetonitrile / water type compared with the GP series by improving classification accuracy of the "Mightysil GP" series. It also can be reliably used for the methanol / water type gradient analysis that cared back pressure. It has good balance of low back pressure and high number of theoretical plates and it is possible to analyze various analysis objects efficiently.
Lower Column Back Pressure
- Column size: 150-4.6 (5 μm)
- Eluent: Acetonitrile/Water=75/25
- Flow rate: 1.0mL/min
- Temp. : 40℃
- Detector: UV254nm
- Inj. Vol.: 5μL
- Sample: Anthracene 5 μg/mL
- Column: Mightysil RP-18MS 150-2 (5 μm) ◆
- Mightysil RP-18GP 150-2 (5 μm) ■
- Flow rate: 0.2mL/min
- Temp.: 40℃
L-column Series
We offer the high-performance HPLC column "L-column series" manufactured by the Chemicals Evaluation and Research Institute, Japan (CERI).
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Product Lineup
● L-column3
L-column3 series has improved chemical durability with highly alkaline resistant PCS silica (perfect chemical stable silica) and perfectly stable end-capping. Therefore, it can be used in a very broad pH range (pH 1 to pH 12). In addition, the low adsorptivity of basic substances, acidic substances and coordination compounds is excellent.
L-column3 is an all-round column that can design any HPLC analytical method freely.
● L-column2
L-column2 series has performance superior to that of L-column series with the new end-capping method. In addtion, this column can support the analysis of a wide range of compounds from acidic to basic substances. L-column2 series is recommended as the first choice column.
● L-column ODS
L-column ODS is a highly versatile reversed-phase HPLC column. This column has excellent separation performance with a new end-capping technology (Patent No. 2611545) using high-temperature silylation.
| Column | Bonded phase | Base silica | End-capping | Particle size(μm) |
|---|---|---|---|---|
| L-column3 C18
|
C18(ODS) | PCS Silica | Yes | 2, 3, 5 |
| L-column3 C8
|
C8(Octyl) | PCS Silica | Yes | 3, 5 |
| L-column2 ODS | C18(ODS) | High purity spherical silica | Yes | 2, 3, 5 |
| L-column2 C8 | C8(Octyl) | High purity spherical silica | Yes | 3, 5 |
| L-column2 C6-Phenyl | Phenyl-Hexyl | High purity spherical silica | Yes | 3, 5 |
| L-column ODS | C18(ODS) | High purity spherical silica | Yes | 3, 5 |
| L-column C8 | C8(Octyl) | High purity spherical silica | Yes | 5 |
| L-column ODS-P | C18(ODS) | High purity spherical silica | Yes | 5 |
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