Cica TEER Measuring System
Cica TEER Measuring System (1CH) is a trans-epithelial electrical resistance (TEER) measurement device for the ad-MED Vitrigel™ series. It can be used to evaluate barrier function in various epithelial and endothelial cells.
Features
1. Stable measurements
The electrode snaps into in the ad-MED Vitrigel™ 2 (12 or 24-well formats). This reduces fluctuations in measured values and enables stable measurements.
2. Continuous measurements
The access port provided on the electrode allows to add the test substance while the electrode remains in place. Continuous measurement using the attached software enables real-time monitoring of the cell layer before and after exposure to the test substance.
What is TEER
Trans-epithelial electrical resistance (TEER or TER) refers to the resistance calculated from the voltage measured when a constant current is applied across a cell layer. Epithelial cells possess a barrier function that separates the internal environment of the body from the external environment. The TEER value is widely used as a non-invasive, real-time indicator for evaluating the barrier integrity of cultured cell layers. TEER measurements are commonly applied to various epithelial cell models, including those of the skin, intestine, nasal cavity, oral mucosa, airway, placenta, kidney, and bladder, as well as vascular endothelial cell models and blood–brain barrier models. TEER measurement is widely used for applications such as verifying the integrity of established cell models and assessing the toxicity or permeability of test substances.
How to use and calculate TEER values
TEER values of the cell layer on the insert is determined by setting the probe portion of the dedicated electrode inside and outside the cell culture insert (ad-MED Vitrigel™ 2) and applying a weak current through the medium and the insert. Prepare a cell culture insert containing cultured cells and a blank insert containing culture medium only (without cells). Place the electrodes in each insert and record the resistance values displayed by the device. The resistance of the blank insert is then subtracted from the total resistance measured for the cell-containing insert (total resistance − blank resistance). The resistance value displayed by the device is expressed in Ω. Multiplying this value by the effective membrane area yields the resistance per unit membrane area (Ω·cm²), which represents the TEER value. *The effective membrane area for ad-MED Vitrigel™ 2 is 1.0 cm² for the 12-well format and 0.33 cm² for the 24-well format.
Product Lineup
| Product No. | Product Name | Package |
|---|---|---|
| 07517-97 | Cica TEER Measuring System (1CH) | 1 |
| 14136-97 | Electrode for TEER Measuring System (12 wells, 1CH) | 1 |
| 14166-97 | Electrode for TEER Measuring System (24 wells, 1CH) | 1 |
| 08363-96 | ad-MED Vitrigel™ 2 (12 well) | 12 pieces/12-well plate |
| 08364-96 | ad-MED Vitrigel™ 2 (24 well) | 24 pieces/24-well plate |
| 08378-96 | ad-MED Vitrigel™ Ex (24 well) | 24 pieces/24-well plate |
Citations
Trabecular Meshwork
- Establishment of appropriate glaucoma models using dexamethasone or TGFβ2 treated three-dimension (3D) cultured human trabecular meshwork (HTM) cells
M.Watanabe, Y.Ida, H.Ohguro, C.Ota, F.Hikage
Scientific reports 11.1 (2021): 19369.
Vascular Endothelium
- Novel microvascular endothelial model utilizing a collagen vitrigel membrane and its advantages for predicting histamine-induced microvascular hyperpermeability
M.Uzu, T.Takezawa
Journal of Pharmacological and Toxicological Methods, 106 (2020). - A hybrid human microvascular endothelial cell line created by utilizing a cell fusion technique and its advantage for expressing high endothelial barrier function
M.Uzu, T.Takezawa
Nano Biomedicine, 14 (1), 9-17 (2022). - Vascular endothelial dysfunction induced by 3-bromofluoranthene via MAPK-mediated-NFκB pro-inflammatory pathway and intracellular ROS generation
Lee CY, Wu SW, Yang JJ, Chen WY, Chen CJ, Chen HH, Lee YC, Su CH, Kuan YH.
Arch Toxicol. 2024 Apr 18. doi: 10.1007/s00204-024-03751-0.
Skin
- Rosae Multiflorae Fructus Extracts Regulate the Differentiation and Vascular Endothelial Cell-mediated Proliferation of Keratinocytes
S.Asayama, T.Igarashi, Y.Abe, A.Iwasaki, M.Kubo, A.Ikeda, K.Akiyama, T.Okamoto, M.Yagi, Y.Niki, H.Ando, M.Ichihashi, K.Mizutani
Bioscience, Biotechnology, and Biochemistry, zbaf007 (2025).
Proximal Tubule Epithelium of the Kidney
- COMMD5 counteracts cisplatin-induced nephrotoxicity by maintaining tubular epithelial integrity and autophagy flux
M.Ogasawara-Nosoko, H.Matsuda, J.Ikeda, M.Abe, Y.Masuhiro, M.Endo, P.Hamet, J.Tremblay
American Journal of Physiology-Renal Physiology, 327.5 (2024).
Airway Epithelium
- The Modulation of Respiratory Epithelial Cell Differentiation by the Thickness of an Electrospun Poly-ε-Carprolactone Mesh Mimicking the Basement Membrane
Choi, S. Y., Kim, H. J., Hwang, S., Park, J., Park, J., Lee, J. W., Son, K. H.
International Journal of Molecular Sciences, 25(12), 6650 (2024).
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