Cica Geneus® Pseudo POT KIT
Pseudomonas aeruginosa is a kind of representative indigenous bacterium that
found naturally in environment. In nosocomial infections, Multiple-Drug-Resistant
Pseudomonas aeruginosa( MDRP) which got tolerance for many antibiotics used to
treat infections become a problem. The PCR-based ORF Typing(POT) is a molecular
epidemiological method to amplify plural specific genes by Multiplex PCR at the
same time, and then to analyze agarose gel electrophoresis banding patterns.
Characteristics
● This POT method developed by Dr. Suzuki of Fujita health University and
Dr.Iinuma of Kanazawa Medical University is a molecular epidemiological
method which can identify and distinguish isolates with strain-level.
● This POT method can compare the homogeny between strains by digitizing
(replace to POT types) an electrocataphoresis result.
● Nearly the same discriminability as the pulsed field gel electrophoresis( PFGE)
and Multilocus Sequence Typing(MLST).
● Required for the examination time is approximately 4 hours.
References
1. Suzuki.M et.al. J.Appl.Microbiol. 101(4):938-947 (2006)
2. Suzuki.M et.al. J.Appl.Microbiol. 107(4):1367-1374 (2009)
Brochure & POT value calculation sheet
Brochure : Cica Geneus Pseudo POT KIT
POT value calculation sheet(Staph)
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