Cica Geneus® ESBL Genotype Detection KIT2
Extended Spectrum β-Lactamase：ESBL is an enzyme hydolyzes wide β-lactam
Extended Spectrum β-Lactamases (ESBLs) are enzymes that mediate resistance to
penicillins, extended-spectrum third generation cephalosporins and monobactams.
This ESBLs-producing bacteria has important clinical and therapeutic implications.
Therefore, the spread of these pathogens puts in peril our antibiotic options.
This kit can detect nine representative ESBLs genotypes. This kit was collaborativery
deveroped by Dr. Ikeda, Dr.Kayama, Dr. Ohge and Dr. Sugai at Hiroshima University.
Characteristics
● This kit can detect representative 9 genotypes for group of ESBLs.
(TEM, SHV, CTX-M-1, CTX-M-2, CTX-M-8, and CTX-M-9 and CTX-M-25, CTX-M chimera, GES (ESBL type) ).
● Required time is approximately 3 hours and suitable for use in common thermal cycler.
● You can use the same PCR condition as our CicaGeneus® AmpC and Carbapenemase genotype detection kit2.
Work Flow
| ① | Isolate ESBL producing bacteria |
|---|---|
| ② | DNA Extraction |
| ③ | Multiplex PCR |
| ④ | Agarose gel electrophoresis |
| ⑤ | Detection |
Target genes and its amplicon size
| Target gene | Amplicon size（bp） | |
|---|---|---|
| Reaction mixture.1 | bla CTX-M-25 group | 522 |
| bla CTX-M chimera | 391 | |
| bla CTX-M-1 group | 268 | |
| bla CTX-M-8 group | 189 | |
| Reaction mixture.2 | bla SHV | 655 |
| bla CTX-M-2 group |
475 | |
| bla CTX-M-9 group | 350 | |
| bla GES (ESBL type) | 228 | |
| bla TEM | 132 |
Example of the electrophoretic pattern
M: 100 bp DNA Ladder, P: Positive control（ Reagent E）,
1: CTX-M-25, 2: CTX-M-64（chimera）, 3: CTX-M-1,
4: CTX-M-8, 5: SHV, 6: CTX-M-2, 7: CTX-M-9,
8: GES-1（ESBL type）, 9: TEM, 10: GES-2（Carbapenemase type）,
N: Negative control（TE Buffer）
Brochure
Cica Geneus ESBL Genotype Detection KIT2
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