Cica Geneus® AmpC Genotype Detection KIT
AmpC producing bacteria, which susceptibility is similar to ESBL-producing bacteria, are
resistant bacteria inhibited by boronic acid, cloxacillin, etc. They are concerned about
becoming ineffective to antibacterial drugs of cephalosporin and cephamycin type.
This kit can detect 6 representative plasmid-mediated AmpC gene family by two
kinds of multiplex PCR.
This kit was collaborativery deveroped by Prof. Dr. Sugai, Assistant Prof. Dr. Kayama
and MMSc. Hara at Hiroshima University.
Characteristics
● This kit can detect 6 AmpC gene family
(CIT family, FOX family, ACT family, DHA family, ACC family, and MOX family).
● Required time is approximately 3 hours.
● Suitable for use in common thermal cycler.
Work Flow
| ① | Isolate AmpC producing bacteria |
|---|---|
| ② | DNA Extraction |
| ③ | Multiplex PCR |
| ④ | Agarose gel electrophoresis |
| ⑤ | Detection |
Target genes and its amplicon size
| Target gene | Amplicon size（bp） | |
|---|---|---|
| Reaction mixture.1 | bla CIT family |
576 |
| bla FOX family |
364 | |
| bla ACT family |
213 | |
| Reaction mixture.2 | bla DHA family |
619 |
| bla ACC family |
403 | |
| bla MOX family |
266 |
Example of the electrophoretic pattern
100：100 bp DNA Ladder, P： Positive control（ Reagent E）,
1：CIT positive bacteria, 2：FOX positive bacteria,
3：ACT positive bacteria, 4：DHA positive bacteria
5：DHA positive bacteria, 6：MOX artificial gene,
N：Negative control（TE Buffer）
Brochure
Cica Geneus AmpC Genotype Detection KIT
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