Cica Geneus Pathogenesis Gene Detection PCR Kit（ for Diarreagenic E.coli）2

Diarrheagenic E. coli is E. coli that causes abdominal pain and diarrhea in humans. It is classified into five types and other
diarrheagenic E. coli based on differences in properties and symptoms. In recent years, there have been frequent cases of
food poisoning and outbreaks caused by Escherichia albertii (E. albertii), closely related to E. coli.
This method of this kit is to amplify plural specific genes by Multiplex PCR at the same time, and then to analyze agarose
gel electrophoresis banding patterns.
This kit can detect the five main types of diarrheagenic E. coli: EHEC/VTEC, EPEC, ETEC, EIEC, and EAggEC, as well
as genes related to the pathogenicity of E. albertii.

Characteristics

■ Using two primer mixes, this kit simultaneously and differentially detects specific genes of E. coli and E. albertii, and seven virulence factors.
*1 Virulence factors: Shiga toxin(Verotoxin) type 1 and 2 genes (stx1, 2), heat-stable toxin (ST) gene (est), heat-labile toxin (LT) gene (elt), intimin gene (eae), agglutination-associated factor (aggR), and enteroinvasive factor (invE).
■ Quantifying detection results allows for clearer interpretation.
■ Included are reagents necessary for PCR, including a positive control.
■ PCR inhibition can be confirmed based on the internal control detection results.

Brochure

Cica Geneus Pathogenesis Gene Detection PCR Kit 2