Cica Geneus® Staph POT KIT

“Passive epidemiological surveillance” is very important to prevent the nosocomial spread of methicillin-resistant Staphylococcus aureus ( MRSA ). For that practical genotyping, PCR-based ORF typing (POT) method is useful.
The PCR-based ORF typing（POT） is a molecular epidemiological method to amplify plural specific genes by multiplex PCR at the same time, for not only investigations of outbreaks but also for non-outbreak epidemiological surveillance.

Characteristics

• This POT method developed by Dr. Suzuki of Fujita health University, it’s a molecular epidemiological method which can identify and distinguish isolates with strain-level.
• This POT method can compare the homogeny between strains by digitizing (replace to POT types) an electrocataphoresis result.
• Nearly the same discriminability as the pulsed field gel electrophoresis（ PFGE）.
• Required for the examination time is approximately 4 hours.

References

1. Suzuki.M et.al. J.Appl.Microbiol. 101(4):938-947 (2006)
2. Suzuki.M et.al. J.Appl.Microbiol. 107(4):1367-1374 (2009)

Brochure & POT value calculation sheet

Brochure : Cica Geneus Staph POT KIT

POT value calculation sheet(Staph)