Cica Geneus® Pseudo POT KIT

Pseudomonas aeruginosa is a kind of representative indigenous bacterium that found naturally in environment. In nosocomial infections, Multiple-Drug-Resistant Pseudomonas aeruginosa（ MDRP） which got tolerance for many antibiotics used to treat infections become a problem. The PCR-based ORF Typing（POT） is a molecular epidemiological method to amplify plural specific genes by Multiplex PCR at the same time, and then to analyze agarose gel electrophoresis banding patterns.

Characteristics

• This POT method developed by Dr. Suzuki of Fujita health University and Dr.Iinuma of Kanazawa Medical University is a molecular epidemiological method which can identify and distinguish isolates with strain-level.
• This POT method can compare the homogeny between strains by digitizing (replace to POT types) an electrocataphoresis result.
• Nearly the same discriminability as the pulsed field gel electrophoresis（ PFGE）and Multilocus Sequence Typing（MLST）.
• Required for the examination time is approximately 4 hours.

References

1. Suzuki.M et.al. J.Appl.Microbiol. 101(4):938-947 (2006)
2. Suzuki.M et.al. J.Appl.Microbiol. 107(4):1367-1374 (2009)

Brochure & POT value calculation sheet

Brochure : Cica Geneus Pseudo POT KIT

POT value calculation sheet(Staph)